Technologies/Resources

Molecular Biology

  • quantitative real time RT-PCR
  • genotyping
  • siRNA knockdown
  • AAV techniques
  • reporter gene assays
  • in situ hybridization
  • western blot

Cell biology

  • cell lines
  • primary cell culture (endothelial cells, neurons, astrocytes, pericytes)
  • slice techniques
  • electrophysiology 
  • FACS
  • calcium imaging

Histology

  • cryoslices
  • paraffin embedding and slicing
  • histological stainings
  • immunohistochemistry
  • fluorescence microscopy
  • confocal microscopy (Leica SP5)

In vivo experiments

  • mouse breeding
  • conditional knockout technique (inducible Cre/lox system)
  • feeding studies (mice, rats)
  • behavioural tests
  • stroke (MCAO)
  • stereotactic injection
  • brain perfusion studies
  • chronic drug treatment
  • two photon microscopy (in collaboration with the Institute for Biomedical Optics, University of Lübeck)
  • electrophysiology (EEG/LFP)
  • sleep studies (humans, mice, rats)
  • transcranial weak electric stimulation
  • amperometry